3D printed mesoporous bioactive glass, bioglass 45S5, and β-TCP scaffolds for regenerative medicine: A comparative in vitro study.

IF 1 4区医学 Q4 ENGINEERING, BIOMEDICAL

Bio-medical materials and engineering Pub Date : 2023-01-01 DOI:10.3233/BME-222524

Maria Jesus Pacheco-Vergara, John L Ricci, Dindo Mijares, Timothy G Bromage, Sasan Rabieh, Paulo G Coelho, Lukasz Witek

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引用次数: 1

Abstract

Background: While autografts to date remain the "gold standard" for bone void fillers, synthetic bone grafts have garnered attention due to their favorable advantages such as ability to be tailored in terms of their physical and chemical properties. Bioactive glass (BG), an inorganic material, has the capacity to form a strong bond with bone by forming a bone-like apatite surface, enhancing osteogenesis. Coupled with additive manufacturing (3D printing) it is possible to maximize bone regenerative properties of the BG.

Objective: The objective of this study was to synthesize and characterize 3D printed mesoporous bioactive glass (MBG), BG 45S5, and compare to β-Tricalcium phosphate (β-TCP) based scaffolds; test cell viability and osteogenic differentiation on human osteoprogenitor cells in vitro.

Methods: MBG, BG 45S5, and β-TCP were fabricated into colloidal gel suspensions, tested with a rheometer, and manufactured into scaffolds using a 3D direct-write micro-printer. The materials were characterized in terms of microstructure and composition with Thermogravimetric Analyzer/Differential Scanning Calorimeter (TGA/DSC), Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Micro-Computed Tomography (μ-CT), Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDS), and Mattauch-Herzog-Inductively Coupled Plasma-Mass Spectrometry (MH-ICP-MS).

Results: Scaffolds were tested for cell proliferation and osteogenic differentiation using human osteoprogenitor cells. Osteogenic media was used for differentiation, and immunocytochemistry for osteogenic markers Runx-2, Collagen-I, and Osteocalcin. The cell viability results after 7 days of culture yielded significantly higher (p < 0.05) results in β-TCP scaffolds compared to BG 45S5 and MBG groups.

Conclusion: All materials expressed osteogenic markers after 21 days of culture in expansion and osteogenic media.

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3D打印介孔生物活性玻璃、生物玻璃45S5和β-TCP再生医学支架的体外比较研究

背景:迄今为止，自体骨移植仍然是骨空隙填充物的“金标准”，而合成骨移植由于其物理和化学性质的可定制性等有利优势而引起了人们的关注。生物活性玻璃(BG)是一种无机材料，能够通过形成骨样磷灰石表面与骨形成牢固的结合，促进成骨。再加上增材制造(3D打印)，可以最大限度地提高BG的骨再生性能。目的:合成并表征3D打印介孔生物活性玻璃(MBG) BG 45S5，并与β-磷酸三钙(β-TCP)基支架进行比较;体外测试人成骨祖细胞的细胞活力和成骨分化。方法:将MBG、BG 45S5和β-TCP制备成胶凝胶悬浮液，用流变仪检测，用3D直写微型打印机制备支架。采用热重分析仪/差示扫描量热仪(TGA/DSC)、傅里叶变换红外光谱(FTIR)、x射线衍射(XRD)、微计算机断层扫描(μ-CT)、扫描电镜(SEM)、能量色散x射线能谱(EDS)和mattauch - herzog -电感耦合等离子体质谱(mah - icp - ms)对材料的微观结构和组成进行了表征。结果:利用人成骨祖细胞对支架进行细胞增殖和成骨分化试验。成骨培养基用于分化，免疫细胞化学用于成骨标志物Runx-2、胶原- i和骨钙素。结论:所有材料在扩增和成骨培养基中培养21天后均表达成骨标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bio-medical materials and engineering 工程技术-材料科学：生物材料

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： The aim of Bio-Medical Materials and Engineering is to promote the welfare of humans and to help them keep healthy. This international journal is an interdisciplinary journal that publishes original research papers, review articles and brief notes on materials and engineering for biological and medical systems. Articles in this peer-reviewed journal cover a wide range of topics, including, but not limited to: Engineering as applied to improving diagnosis, therapy, and prevention of disease and injury, and better substitutes for damaged or disabled human organs; Studies of biomaterial interactions with the human body, bio-compatibility, interfacial and interaction problems; Biomechanical behavior under biological and/or medical conditions; Mechanical and biological properties of membrane biomaterials; Cellular and tissue engineering, physiological, biophysical, biochemical bioengineering aspects; Implant failure fields and degradation of implants. Biomimetics engineering and materials including system analysis as supporter for aged people and as rehabilitation; Bioengineering and materials technology as applied to the decontamination against environmental problems; Biosensors, bioreactors, bioprocess instrumentation and control system; Application to food engineering; Standardization problems on biomaterials and related products; Assessment of reliability and safety of biomedical materials and man-machine systems; and Product liability of biomaterials and related products.