Optimization of a Validated Protocol That Determines Bioavailable Fluoride in Toothpastes.

IF 2.9 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Caries Research Pub Date : 2023-01-01 Epub Date: 2023-08-01 DOI:10.1159/000533154

Luis Fernando Bandeira Miranda, Cinthia Pereira Machado Tabchoury, Jaime Aparecido Cury

引用次数: 0

Abstract

A validated protocol used to determine how much of total fluoride is bioavailable in toothpaste formulations was evaluated if it could be simplified regarding the following parameters: (1) concentration of the slurry (0.25-4%), (2) centrifugation speed (1,000-12,000 g) and time (1-10 min), and (3) incubation time for FPO32- ion hydrolysis and insoluble-F dissolution (15-60 min). Fresh and aged Na2FPO3/CaCO3 and fresh NaF/SiO2-based toothpastes were analyzed (n = 9). The improvements were the centrifugation at 1,000 g for 5 min is enough to separate insoluble-F and the time for FPO32- ion hydrolysis and insoluble-F dissolution can be shortened to 30 min.

查看原文本刊更多论文

优化测定牙膏中生物可利用氟的验证方案。

我们对用于确定牙膏配方中生物可利用的总氟化物含量的有效方案进行了评估，以确定是否可以简化以下参数：(1) 泥浆浓度（0.25-4%）；(2) 离心速度（1,000-12,000 g）和时间（1-10 分钟）；(3) FPO32 离子水解和不溶性-F 溶解的孵育时间（15-60 分钟）。对新鲜和老化的 Na2FPO3/CaCO3 和新鲜的 NaF/SiO2 型牙膏进行了分析（n = 9）。结果表明，1,000 g 离心 5 分钟足以分离不溶性-F，FPO32- 离子水解和不溶性-F 溶解的时间可缩短至 30 分钟。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Caries Research 医学-牙科与口腔外科

CiteScore

6.30

自引率

7.10%

发文量

审稿时长

6-12 weeks

期刊介绍： ''Caries Research'' publishes epidemiological, clinical and laboratory studies in dental caries, erosion and related dental diseases. Some studies build on the considerable advances already made in caries prevention, e.g. through fluoride application. Some aim to improve understanding of the increasingly important problem of dental erosion and the associated tooth wear process. Others monitor the changing pattern of caries in different populations, explore improved methods of diagnosis or evaluate methods of prevention or treatment. The broad coverage of current research has given the journal an international reputation as an indispensable source for both basic scientists and clinicians engaged in understanding, investigating and preventing dental disease.