Presence of methanogenic archaea in necrotic root canals of patients with or without type 2 diabetes mellitus

IF 1.3 4区医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Australian Endodontic Journal Pub Date : 2023-09-15 DOI:10.1111/aej.12797

Ana I. Soza-Bolaños DDS, Endod, Rubén A. Domínguez-Pérez DDS, MSc, Endod, PhD, José L. Ayala-Herrera DDS, MSc, PhD, Rosa M. Pérez-Serrano PhD, Uriel Soto-Barreras DDS, MSc, PhD, León F. Espinosa-Cristóbal DDS, MSc, PhD, Claudia A. Rivera-Albarrán DDS, MSc, PhD, Guadalupe Zaldívar-Lelo de Larrea MD, MSc, PhD

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引用次数: 0

Abstract

Theoretically, a necrotic root canal fulfils all requirements as a niche for methanogens to inhabit. However, their presence in it and its implication in apical periodontitis (AP) is controversial. Therefore, to contribute to ending the controversy, this study aimed to detect and compare methanogens' presence in two distinct niches with supposedly different microenvironments; both were necrotic root canals associated with AP but one from patients with type 2 diabetes mellitus (T2DM) while the other from non-diabetic patients. A clinical examination was performed on 65 T2DM patients and 73 non-diabetic controls. Samples from necrotic root canals were obtained, and methanogens were identified. The presence of methanogens was three times higher (27.6%) in the T2DM group than in non-diabetic patients (8.2%). In addition, methanogens' presence was associated with a higher prevalence of periapical symptoms.

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2 型糖尿病患者或非 2 型糖尿病患者坏死根管中的产甲烷古菌

从理论上讲，坏死的根管符合甲烷菌栖息的所有要求。然而，它们在根管中的存在及其对根尖牙周炎（AP）的影响还存在争议。因此，为了结束这一争议，本研究旨在检测和比较甲烷菌在两个不同的壁龛中的存在情况，这两个壁龛的微环境应该是不同的；两者都是与根尖周炎相关的坏死根管，但一个来自 2 型糖尿病（T2DM）患者，另一个来自非糖尿病患者。对 65 名 T2DM 患者和 73 名非糖尿病对照组患者进行了临床检查。从坏死的根管中提取样本，并对甲烷菌进行鉴定。T2DM 组中甲烷菌的存在率（27.6%）是非糖尿病患者（8.2%）的三倍。此外，甲烷菌的存在与根尖周症状的发生率较高有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Australian Endodontic Journal DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.50

自引率

6.20%

发文量

审稿时长

>12 weeks

期刊介绍： The Australian Endodontic Journal provides a forum for communication in the different fields that encompass endodontics for all specialists and dentists with an interest in the morphology, physiology, and pathology of the human tooth, in particular the dental pulp, root and peri-radicular tissues. The Journal features regular clinical updates, research reports and case reports from authors worldwide, and also publishes meeting abstracts, society news and historical endodontic glimpses. The Australian Endodontic Journal is a publication for dentists in general and specialist practice devoted solely to endodontics. It aims to promote communication in the different fields that encompass endodontics for those dentists who have a special interest in endodontics.