Surface grafting of polymeric catheters and stents to prevent biofilm formation of pathogenic bacteria.

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Reham G Elfarargy, Mohamed Sedki, Farag A Samhan, Rabeay Y A Hassan, Ibrahim M El-Sherbiny
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Abstract

Background: Tecothane (medical grade of polyurethane) is strongly involved in the fabrication of metallic and polymeric-based medical devices (e.g., catheters and stents) as they can withstand cardiac cycle-related forces without deforming or failing, and they can mimic tissue behavior. The main problem is microbial contamination and formation of pathogenic biofilms on such solid surfaces within the human body. Accordingly, our hypothesis is the coating of tecothane outer surfaces with antibacterial agents through the electro-deposition or chemical grafting of anti-biofilm agents onto the stent and catheter surfaces.

Results: Tecothane is grafted with itaconic acid for cross-linking the polyethyleneimine (PEI) as the protective-active layer. Accordingly, the grafting of poly-itaconic acid onto the Tecothane was achieved by three different methods: wet-chemical approach, electro-polymerization, or by using plasma treatment. The successful modifications were verified using Fourier Transform Infrared (FTIR) spectroscopy, grafting percentage calculations, electrochemical, and microscopic monitoring of biofilm formation. The grafting efficiency of itaconic acid was over 3.2% (w/w) at 60 ℃ after 6 h of the catheter chemical modification. Bio-electrochemical signals of biofilms have been seriously reduced after chemical modification because of the inhibition of biofilm formation (for both Pseudomonas aeruginosa and Staphylococcus aureus) over a period of 9 days.

Conclusion: Chemical functionalization of the polyurethane materials with the antimicrobial and anti-biofilm agents led to a significant decrease in the formation of pathogenic biofilms. This promising proof-concept will open the door to explore further surface protection with potential anti-biofilm agents providing better and sustainable productions of stents and catheters biomaterials.

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高分子导管和支架表面接枝,防止致病菌形成生物膜。
背景:Tecothane(医用级聚氨酯)在金属和聚合物基医疗器械(例如,导管和支架)的制造中发挥着重要作用,因为它们可以承受心脏周期相关的力而不会变形或失效,并且它们可以模拟组织行为。主要问题是微生物污染和在人体内这种固体表面形成致病生物膜。因此,我们的假设是通过电沉积或化学接枝将抗生物膜剂涂在支架和导管表面,在tecothane外表面涂上抗菌剂。结果:以衣康酸接枝Tecothane交联聚乙烯亚胺(PEI)作为保护活性层。因此,聚衣康酸通过三种不同的方法接枝到Tecothane上:湿化学方法、电聚合或等离子体处理。通过傅里叶变换红外光谱(FTIR)、接枝百分比计算、电化学和生物膜形成的微观监测验证了成功的修饰。经60℃化学修饰6 h后,衣康酸接枝效率可达3.2% (w/w)以上。化学修饰后生物膜的电化学信号严重降低,因为在9天的时间内抑制了生物膜的形成(无论是铜绿假单胞菌还是金黄色葡萄球菌)。结论:抗菌和抗生物膜剂对聚氨酯材料的化学功能化作用可显著减少致病性生物膜的形成。这一有希望的概念验证将为探索潜在的抗生物膜剂的进一步表面保护打开大门,提供更好和可持续的支架和导管生物材料生产。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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