Kidney morphology and renal expression of aquaporins 2, 3 and 4 during cerulein – Induced chronic pancreatitis in pigs

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences Pub Date : 2023-09-01 DOI:10.1016/j.advms.2023.09.002

Katarzyna Michałek , Patrycja Oberska , Maciej Murawski , Tomasz Schwarz , Ewa Tomaszewska , Siemowit Muszyński , Małgorzata Świątkiewicz , Łukasz Korytkowski , Joanna Bonior , Mateusz Zelent , David Salako-Adeoye Ayomide , Marta Grabowska

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引用次数: 0

Abstract

Purpose

Chronic pancreatitis (CP) is associated with serious complications and reduced quality of life. Kidney failure is a frequent complication of acute pancreatitis (AP), however limited information is available regarding the impact of CP on this condition. In the kidney, 9 aquaporins (AQPs) are expressed to maintain body water homeostasis and concentrate urine. The purpose of this study was to morphologically assess and analyze the location and expression of AQP2, AQP3 and AQP4 and determine whether CP affects renal structure and expression of AQPs in collecting duct (CD) principal cells.

Materials/methods

CP was induced in domestic pigs through intramuscular injections of cerulein (1 μg/kg bw/day for 6 days; n = 5); pigs without CP (n = 5) were used as a control group. Kidney samples were collected 6 weeks after the last injection and subjected to histological examination. Expression of AQPs was determined by immunohistochemistry and Western blot.

Results

The kidneys of animals with CP exhibited moderate changes, including glomerular enlargement, increased collagen percentage, numerous stromal erythrorrhages and inflammatory infiltrations compared to control group. Although the total abundance of AQP2 in the CD decreased in pigs after cerulein administration, the difference was not statistically significant. Expression of AQP3 and AQP4 was limited to the basolateral membrane of the CD cells. AQP4 abundance remained relatively stable in both groups, while AQP3 expression increased nearly three-fold in pigs with CP.

Conclusion

This study identified morphological alterations and a statistically significant increase in the expression of renal AQP3 when pigs developed CP.

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猪慢性胰腺炎猪肾形态及水通道蛋白2、3和4的表达

目的慢性胰腺炎（CP）与严重并发症和生活质量下降有关。肾衰竭是急性胰腺炎（AP）的常见并发症，但关于CP对这种情况的影响，目前的信息有限。在肾脏中，9种水通道蛋白（AQPs）被表达以维持体内水分稳态并浓缩尿液。本研究的目的是从形态学上评估和分析AQP2、AQP3和AQP4的位置和表达，并确定CP是否影响肾结构和收集管（CD）主细胞中AQPs的表达。材料/方法家猪通过肌肉注射天蓝素（1μg/kgbw/天，持续6天；n=5）；无CP的清管器（n=5）作为对照组。最后一次注射后6周采集肾脏样本，并进行组织学检查。免疫组织化学和蛋白质印迹法检测AQPs的表达。结果与对照组相比，CP动物的肾脏表现出中度变化，包括肾小球增大、胶原百分比增加、大量基质红细胞出血和炎症浸润。尽管在施用天蓝色素后，猪CD中AQP2的总丰度降低，但差异无统计学意义。AQP3和AQP4的表达仅限于CD细胞的基底外侧膜。AQP4的丰度在两组中都保持相对稳定，而AQP3的表达在患有CP的猪中增加了近三倍。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Advances in medical sciences 医学-医学：研究与实验

CiteScore

5.00

自引率

0.00%

发文量

审稿时长

25 days

期刊介绍： Advances in Medical Sciences is an international, peer-reviewed journal that welcomes original research articles and reviews on current advances in life sciences, preclinical and clinical medicine, and related disciplines. The Journal’s primary aim is to make every effort to contribute to progress in medical sciences. The strive is to bridge laboratory and clinical settings with cutting edge research findings and new developments. Advances in Medical Sciences publishes articles which bring novel insights into diagnostic and molecular imaging, offering essential prior knowledge for diagnosis and treatment indispensable in all areas of medical sciences. It also publishes articles on pathological sciences giving foundation knowledge on the overall study of human diseases. Through its publications Advances in Medical Sciences also stresses the importance of pharmaceutical sciences as a rapidly and ever expanding area of research on drug design, development, action and evaluation contributing significantly to a variety of scientific disciplines. The journal welcomes submissions from the following disciplines: General and internal medicine, Cancer research, Genetics, Endocrinology, Gastroenterology, Cardiology and Cardiovascular Medicine, Immunology and Allergy, Pathology and Forensic Medicine, Cell and molecular Biology, Haematology, Biochemistry, Clinical and Experimental Pathology.