Regulation of vagally-evoked respiratory responses by the lateral parabrachial nucleus in the mouse

IF 1.9 4区医学 Q3 PHYSIOLOGY

Respiratory Physiology & Neurobiology Pub Date : 2023-10-01 DOI:10.1016/j.resp.2023.104141

Robert Behrens , Mathias Dutschmann , Matthew Trewella , Stuart B. Mazzone , Aung Aung Kywe Moe

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Abstract

Vagal sensory inputs to the brainstem can alter breathing through the modulation of pontomedullary respiratory circuits. In this study, we set out to investigate the localised effects of modulating lateral parabrachial nucleus (LPB) activity on vagally-evoked changes in breathing pattern. In isoflurane-anaesthetised and instrumented mice, electrical stimulation of the vagus nerve (eVNS) produced stimulation frequency-dependent changes in diaphragm electromyograph (dEMG) activity with an evoked tachypnoea and apnoea at low and high stimulation frequencies, respectively. Muscimol microinjections into the LPB significantly attenuated eVNS-evoked respiratory rate responses. Notably, muscimol injections reaching the caudal LPB, previously unrecognised for respiratory modulation, potently modulated eVNS-evoked apnoea, whilst muscimol injections reaching the intermediate LPB selectively modulated the eVNS-evoked tachypnoea. The effects of muscimol on eVNS-evoked breathing rate changes occurred without altering basal eupneic breathing. These results highlight novel roles for the LPB in regulating vagally-evoked respiratory reflexes.

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小鼠臂旁外侧核对迷走神经诱发呼吸反应的调节

脑干的迷走神经感觉输入可以通过调节脑干旁呼吸回路来改变呼吸。在这项研究中，我们着手研究调节臂旁外侧核（LPB）活动对迷走神经诱发的呼吸模式变化的局部影响。在异氟烷麻醉和仪器化的小鼠中，迷走神经（eVNS）的电刺激分别在低和高刺激频率下产生膈肌肌电图（dEMG）活动的刺激频率依赖性变化，诱发呼吸急促和呼吸暂停。肌肉注射到LPB中显著减弱了eVNS诱发的呼吸频率反应。值得注意的是，到达尾部LPB的肌肉注射（以前未被识别为呼吸调节）有效地调节了eVNS诱发的呼吸暂停，而到达中间LPB的肌注射选择性地调节了eVNS诱发的快速呼吸。麝香醇对eVNS诱发的呼吸频率变化的影响发生在不改变基础呼吸的情况下。这些结果突出了LPB在调节迷走神经诱发的呼吸反射中的新作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Respiratory Physiology & Neurobiology 医学-呼吸系统

CiteScore

4.80

自引率

8.70%

发文量

104

审稿时长

54 days

期刊介绍： Respiratory Physiology & Neurobiology (RESPNB) publishes original articles and invited reviews concerning physiology and pathophysiology of respiration in its broadest sense. Although a special focus is on topics in neurobiology, high quality papers in respiratory molecular and cellular biology are also welcome, as are high-quality papers in traditional areas, such as: -Mechanics of breathing- Gas exchange and acid-base balance- Respiration at rest and exercise- Respiration in unusual conditions, like high or low pressure or changes of temperature, low ambient oxygen- Embryonic and adult respiration- Comparative respiratory physiology. Papers on clinical aspects, original methods, as well as theoretical papers are also considered as long as they foster the understanding of respiratory physiology and pathophysiology.