The association of IL-17A rs2275913 single nucleotide polymorphism with anti-tuberculous drug resistance in patients with pulmonary tuberculosis.

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Asmaa A Elmadbouly, Abeer Mohammed Abdul-Mohymen, Heba H Eltrawy, Hanaa A Abou Elhasan, Azza Ali Althoqapy, Doaa R Amin
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Abstract

Background: Drug-resistant  Tuberculosis (DR-TB) is a global health burden with high morbidity and mortality in developing countries including Egypt. The susceptibility to infection with DR-TB strains may be genetically determined. Several interleukin gene polymorphisms were investigated as risk factors for tuberculosis infection but focusing on their association with DR-TB was limited. Therefore, the objective of this study is to assess the association of IL 17 - 197 G > A (rs2275913) single nucleotide polymorphism (SNP) with susceptibility to DR-TB strains in comparison to drug-sensitive tuberculosis (DS-TB) strains in Egyptian patients with pulmonary TB. This cross-sectional study was conducted on 80 patients with DR-TB strains and 80 with DS-TB strains as a control group. Both age and sex were comparable among the study's groups. IL-17 - 197 G > A (rs2275913) SNP was genotyped by real-time PCR, and IL-17 serum concentration was measured by enzyme-linked immunosorbent assay (ELISA).

Results: The GA and AA genotype frequencies of IL 17 - 197 G > A (rs2275913) SNP were significantly higher in patients with DR-TB strains than those with DS-TB strains (p < 0.001). The frequency of the A allele was significantly (p < 0.001) higher in patients with DR-TB group (32.5%) compared to the control group (13.8%). Substantial higher serum levels of IL-17 were detected in the DR-TB group with significant association with AA and AG genotypes.

Conclusion: Polymorphism in IL-17 -197 G > A (rs2275913) resulted in higher serum levels of IL-17 and Egyptian patients with such polymorphism are three times at risk of infection with DR-TB strains than patients with wild type.

Abstract Image

IL-17A rs2275913单核苷酸多态性与肺结核患者抗结核耐药的关系
背景:耐药结核病(DR-TB)是一种全球卫生负担,在包括埃及在内的发展中国家具有高发病率和死亡率。对耐药结核菌株感染的易感性可能由遗传决定。研究了几种白细胞介素基因多态性作为结核病感染的危险因素,但对其与耐药结核病的关系的关注有限。因此,本研究的目的是评估IL 17 - 197 G b> A (rs2275913)单核苷酸多态性(SNP)与埃及肺结核患者耐药结核菌株和药敏结核(DS-TB)菌株易感性的关系。本横断面研究以80例DR-TB患者和80例DS-TB患者为对照组。研究小组的年龄和性别都具有可比性。实时荧光定量PCR检测IL-17 - 197 G > A (rs2275913) SNP基因型,酶联免疫吸附试验(ELISA)检测血清IL-17浓度。结果:IL-17 -197 G > A (rs2275913) SNP的GA和AA基因型频率在耐药结核患者中显著高于DS-TB患者(p结论:IL-17 -197 G > A (rs2275913)多态性导致血清IL-17水平升高,具有该多态性的埃及患者感染耐药结核的风险是野生型患者的3倍。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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