Detection of Antibodies against Toxoplasma from Human Serum Sample using ELISA.

Q3 Pharmacology, Toxicology and Pharmaceutics

Infectious disorders drug targets Pub Date : 2023-01-01 DOI:10.2174/1871526522666220913152447

Kirtika Sharma, Saumya Srivastava, Aditya Kundu, Vibhor Tak

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引用次数: 0

Abstract

Background: Toxoplasmosis is a common worldwide zoonotic infection affecting warm blooded animals and humans caused by Toxoplasma gondii. Clinical features range from mildfebrile illness and lymphadenopathy in the immunocompetent host to encephalitisin the immunosuppressed host (E.g. HIV infected patients). Congenital infection can also occur. For effective control and treatment of toxoplasmosis, accurate detection of T. gondii infection is important.

Objective: In this study, ELISA detecting anti-toxoplasma antibodies IgM and IgG has been used for diagnosing Toxoplasmosis in patients.

Methods: Enzyme-linked immunosorbentassay (ELISA) was carried out in serum samples collected from 40 patients to detect anti-toxoplasma IgG and IgM antibodies as a part of work up in suspected cases. Relevant clinical history was also taken.

Results: Of the total 40 samples taken, only one sample came positive for IgM and 9 came positive for IgG antibody. All patients who were seropositivefor T. gondii antibodies had HIV infection. Five patients were in the age group between 30 to 40 years.

Conclusion: From the limited data available in this study, it may be recommended to screen for T. gondii antibodies in HIV patients.

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ELISA法检测人血清弓形虫抗体。

背景:弓形虫病是由刚地弓形虫引起的一种影响温血动物和人类的常见人畜共患传染病。临床特征从免疫正常宿主的轻度发热和淋巴结病变到免疫抑制宿主(如HIV感染患者)的脑炎。先天性感染也可能发生。弓形虫感染的准确检测是有效控制和治疗弓形虫病的重要手段。目的:采用ELISA法检测弓形虫抗体IgM和IgG，对弓形虫病进行诊断。方法:采用酶联免疫吸附试验(ELISA)检测40例疑似病例血清中抗弓形虫IgG和IgM抗体。同时采集相关临床病史。结果:40份样本中，IgM阳性1份，IgG抗体阳性9份。所有血清弓形虫抗体阳性的患者均为HIV感染。5例患者年龄在30至40岁之间。结论:根据本研究有限的数据，建议对HIV患者进行弓形虫抗体筛查。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Infectious disorders drug targets Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

3.10

自引率

0.00%

发文量

123

期刊介绍： Infectious Disorders - Drug Targets aims to cover all the latest and outstanding developments on the medicinal chemistry, pharmacology, molecular biology, genomics and biochemistry of contemporary molecular targets involved in infectious disorders e.g. disease specific proteins, receptors, enzymes, genes. Each issue of the journal contains a series of timely in-depth reviews written by leaders in the field covering a range of current topics on drug targets involved in infectious disorders. As the discovery, identification, characterization and validation of novel human drug targets for anti-infective drug discovery continues to grow, this journal will be essential reading for all pharmaceutical scientists involved in drug discovery and development.