Identification and validation of novel Blastocystis subtype ST41 in a Colombian patient undergoing colorectal cancer screening

IF 2.1 4区生物学 Q3 MICROBIOLOGY

Journal of Eukaryotic Microbiology Pub Date : 2023-05-17 DOI:10.1111/jeu.12978

Carolina Hernández-Castro, Jenny G. Maloney, Sonia P. Agudelo-López, Miguel A. Toro-Londoño, Jorge H. Botero-Garcés, María C. Orozco, Yulieth C. Quintero-Quinchia, Juan C. Correa-Cote, Alejandro Múnera-Duque, Juan C. Ricaurte-Ciro, Luis I. Londoño-Álvarez, René M. Escobar, Pamela C. Köster, Sergio Sánchez, David Carmena, Mónica Santín

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引用次数: 3

Abstract

Blastocystis sp. is among the most frequent intestinal protists identified in humans globally. However, characterization of Blastocystis subtype diversity in humans is ongoing. We report here the identification of novel Blastocystis subtype ST41 in a Colombian patient undergoing colorectal cancer screening involving colonoscopy and fecal testing (microscopy, culture, PCR). The full-length ssu rRNA gene sequence of the protist was generated using MinION long-read sequencing technology. The validity of the novel subtype was confirmed via phylogenetic and pairwise distance analyses of the full-length ST41 sequence and all other valid subtypes. The study provides reference material essential for conducting subsequent experimental studies.

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在接受结直肠癌筛查的哥伦比亚患者中鉴定和验证新型囊虫亚型ST41

囊虫是全球最常见的人类肠道原生生物之一。然而，人类囊虫亚型多样性的表征仍在进行中。我们在此报告一名哥伦比亚患者在结肠直肠癌筛查中发现了新型囊虫ST41亚型，包括结肠镜检查和粪便检测(显微镜、培养、PCR)。使用MinION长读测序技术生成该原生生物的ssu rRNA基因全长序列。通过对ST41全长序列和所有其他有效亚型的系统发育和配对距离分析，证实了新亚型的有效性。本研究为后续实验研究提供了必要的参考资料。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Eukaryotic Microbiology 生物-微生物学

CiteScore

4.30

自引率

4.50%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal of Eukaryotic Microbiology publishes original research on protists, including lower algae and fungi. Articles are published covering all aspects of these organisms, including their behavior, biochemistry, cell biology, chemotherapy, development, ecology, evolution, genetics, molecular biology, morphogenetics, parasitology, systematics, and ultrastructure.