Protection provided by a commercial modified-live porcine reproductive and respiratory syndrome virus (PRRSV) 1 vaccine (PRRSV1-MLV) against a Japanese PRRSV2 field strain.

IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES
Journal of Veterinary Science Pub Date : 2023-09-01 Epub Date: 2023-07-07 DOI:10.4142/jvs.23025
Joel Miranda, Salvador Romero, Lidia de Lucas, Fumitoshi Saito, Mar Fenech, Ivan Díaz
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引用次数: 0

Abstract

Background: Porcine reproductive and respiratory syndrome virus (PRRSV) vaccines do not provide full cross-protection, mainly due to the virus genetic variability. Despite this, vaccines based on modified-live PRRSV (PRRSV-MLV) reduce the disease impact.

Objectives: To assess the efficacy of two commercial vaccines-one based on PRRSV1 (PRRSV1-MLV) and another on PRRSV2 (PRRSV2-MLV)-against a Japanese PRRSV2 field strain.

Methods: Two groups of three-week-old piglets were vaccinated (G1: PRRSV1-MLV; G2: PRRSV2-MLV) and two were kept as non-vaccinated (INF and CTRL). One month later, G1, G2, and INF were challenged with a PRRSV2 field strain.

Results: After the challenge, clinical signs were only observed in INF. Moreover, the highest rectal temperatures and values for the area under the curve (AUC) were observed in INF. Regarding viral detection, both AUC and the proportion of positive samples in blood were higher in INF. In G1, viremic animals never reached 100%. At necropsy (21 d after the challenge), differences for titers among groups were only found in tonsils (G1 < G2 and INF). One animal (belonging to G1) was negative in all tissues. Regarding humoral responses, G1 and G2 seroconverted after vaccination, as detected in the corresponding enzyme-linked immunosorbent assay. Specific neutralizing antibodies (NA) against PRRSV1-MLV were already detected at 14 d after vaccination in G1, showing a significant booster after the challenge, while PRRSV2-MLV NA were detected in G2 at the end of the experiment.

Conclusions: Despite genetic differences, PRRSV1-MLV has been demonstrated to confer partial protection against a Japanese PRRSV2 strain, at least as good as PRRSV2-MLV.

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由商业改良的猪繁殖和呼吸综合征活病毒(PRRSV)1疫苗(PRRSV1-MLV)对日本PRRSV2现场株提供的保护。
背景:猪繁殖与呼吸综合征病毒(PRRSV)疫苗不能提供完全的交叉保护,主要是由于病毒的遗传变异性。尽管如此,基于改良活PRRSV(PRRSV-MLV)的疫苗降低了疾病的影响。目的:评估两种商业疫苗(一种基于PRRSV1(PRRSV1-MLV))对日本PRRSV2现场株的效力。方法:两组三周龄仔猪接种疫苗(G1:PRRRSV1-MLV;G2:PRRRSV2-MLV),两组未接种疫苗(INF和CTRL)。一个月后,用PRRSV2现场菌株攻击G1、G2和INF。结果:激发后,仅在INF中观察到临床症状。此外,在INF中观测到最高的直肠温度和曲线下面积(AUC)值。关于病毒检测,INF中的AUC和血液中阳性样本的比例都更高。在G1期,病毒血症动物从未达到100%。尸检时(激发后21天),各组之间的滴度仅在扁桃体中发现差异(G1<G2和INF)。一只动物(属于G1)的所有组织均为阴性。关于体液反应,G1和G2在接种疫苗后血清转化,如在相应的酶联免疫吸附测定中检测到的。在G1期接种疫苗后14天,已经检测到针对PRRSV1-MLV的特异性中和抗体(NA),显示出攻击后的显著增强,而在实验结束时,在G2期检测到PRRSV2-MLV NA。结论:尽管存在遗传差异,但PRRSV1-MLV已被证明对日本PRRSV2株具有部分保护作用,至少与PRRSV2-MLV一样好。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Veterinary Science
Journal of Veterinary Science 农林科学-兽医学
CiteScore
3.10
自引率
5.60%
发文量
86
审稿时长
1.3 months
期刊介绍: The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.
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