EXPRESSION OF HEPATOCYTE GROWTH FACTOR AND C-MET RECEPTOR IN STROMAL FIBROBLASTS AND TUMOR CELLS OF ENDOMETRIAL CARCINOMA.

Q3 Medicine

Experimental oncology Pub Date : 2023-06-26 DOI:10.15407/exp-oncology.2023.01.079

L G Buchynska, O V Brieieva, S V Nespriadko

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引用次数: 0

Abstract

Background: HGF/c-Met is one of the main signaling pathways that ensure communication between epithelial cells and components of the tumor microenvironment determining the invasive and metastatic potential of many cancers. However, the significance of HGF and c-Met in endometrial carcinoma (ECa) progression remains unclear.

Aim: To evaluate copy number variations as well as expression of the c-Met receptor and its ligand HGF in endometrial carcinomas considering the clinical and morphological characteristics of ECa.

Materials and methods: The study was conducted on ECa samples of 57 patients, among which 32 had lymph nodes and/or distant metastasis. The copy number of c-MET gene was estimated by qPCR. The expression of HGF and c-Met in tissue samples was determined by the immunohistochemical method.

Results: Amplification of c-MET gene was detected in 10.5% of the ECa cases. In most carcinomas, a combined expression pattern of HGF and c-Met was established, in which co-expression of these markers was observed in tumor cells, and the content of HGF+ fibroblasts increased in the stroma. The expression of HGF in tumor cells was associated with the tumor differentiation grade and was higher in G3 ECa (p = 0.041). The number of HGF+ fibroblasts in the stromal component increased in the ECa cases with metastasis compared to the cases without metastasis (p = 0.032). The content of stromal c-Met+ fibroblasts was higher in deeply invasive carcinomas of patients with metastases than in tumors with invasion of < 1/2 myometrium (p = 0.035).

Conclusion: Increased expression of HGF and c-Met in stromal fibroblasts of endometrial carcinomas is associated with metastasis in patients with ECa and deep invasion of the tumor into the myometrium, and can contribute to the aggressive course of the disease.

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肝细胞生长因子和c-met受体在子宫内膜癌间质成纤维细胞和肿瘤细胞中的表达。

背景:HGF/c-Met是确保上皮细胞和肿瘤微环境成分之间通信的主要信号通路之一，决定了许多癌症的侵袭和转移潜力。然而，HGF和c-Met在子宫内膜癌(ECa)进展中的意义尚不清楚。目的:结合ECa的临床和形态学特点，探讨c-Met受体及其配体HGF在子宫内膜癌中的拷贝数变化及表达。材料和方法:本研究对57例患者的ECa样本进行研究，其中32例有淋巴结和/或远处转移。采用qPCR法估计c-MET基因拷贝数。免疫组化法检测组织样品中HGF和c-Met的表达。结果:10.5%的ECa患者检测到c-MET基因扩增。在大多数肿瘤中，HGF和c-Met的联合表达模式被建立，在肿瘤细胞中观察到这两种标记物的共表达，并且间质中HGF+成纤维细胞的含量增加。HGF在肿瘤细胞中的表达与肿瘤分化程度相关，在G3 ECa中表达较高(p = 0.041)。有转移的ECa患者间质成分中HGF+成纤维细胞数量明显高于无转移的ECa患者(p = 0.032)。深浸润癌转移患者间质c-Met+成纤维细胞含量高于浸润< 1/2肌层的肿瘤(p = 0.035)。结论:子宫内膜癌间质成纤维细胞中HGF和c-Met的表达升高与ECa患者的转移和肿瘤向肌层的深度浸润有关，并可能促进疾病的侵袭性发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Experimental oncology Medicine-Oncology

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： The Experimental Oncology is an English-language journal that publishes review articles, original contributions, short communications, case reports and technical advances presenting new data in the field of experimental and fundamental oncology. Manuscripts should be written in English, contain original work, which has not been published or submitted for publication elsewhere. It also implies the transfer of the Copyright from the author to “Experimental Oncology”. No part of journal publications may be reproduced, stored in a retrieval system or transmitted in any form or by any means without the prior permission of the publisher.