Detection of blaCTX-M genes in ESBL-producing Klebsiella isolates from animals in Croatia.

IF 0.7 4区 农林科学 Q3 VETERINARY SCIENCES
Selma Pintarić, Zrinka Štritof, Vesna Mojčec Perko, Andrea Tumpa, Marija Cvetnić, Lana Hadžić
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引用次数: 0

Abstract

This study investigated the frequency of third-generation cephalosporin resistance in Klebsiella spp. isolated from animals in Croatia and the presence of blaCTX-M genes. A total of 711 enteric bacteria were isolated from clinical samples, and Klebsiella spp. isolates accounted for 6.9% (n = 49). Thirteen Klebsiella isolates (26.5%) were ESBL producers, nine isolates from the Klebsiella pneumoniae species complex (69.2%), and four (30.8%) Klebsiella oxytoca isolates. All carried the blaCTX-M-15 gene, and antimicrobial susceptibility testing revealed them as multidrug resistant. All were resistant to all tested cephalosporins, fluoroquinolones, aminoglycosides and aztreonam, 92.3% showed resistance to tetracycline, 84.6% to trimethoprim-sulfamethoxazole and 69.2% to nitrofurantoin. No isolate showed resistance to imipenem and meropenem. It can be concluded that ESBL-producing Klebsiella expressing the blaCTX-M gene are not rare among Klebsiella isolates from animals in Croatia.

克罗地亚产esbl克雷伯菌分离株中blaCTX-M基因的检测
本研究调查了从克罗地亚动物中分离的克雷伯氏菌属第三代头孢菌素耐药的频率和blaCTX-M基因的存在。临床标本共检出肠道细菌711株,其中克雷伯氏菌占6.9% (n = 49)。产生ESBL的克雷伯菌13株(26.5%),肺炎克雷伯菌复合体9株(69.2%),产氧克雷伯菌4株(30.8%)。所有菌株均携带blaCTX-M-15基因,药敏试验显示它们具有多重耐药。对所有头孢菌素类、氟喹诺酮类、氨基糖苷类和氨曲南耐药,对四环素耐药92.3%,对甲氧苄啶-磺胺甲恶唑耐药84.6%,对呋喃妥英耐药69.2%。没有分离株对亚胺培南和美罗培南耐药。由此可见,在克罗地亚动物分离的克雷伯菌中,表达blaCTX-M基因的产esbl克雷伯菌并不罕见。
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来源期刊
Acta veterinaria Hungarica
Acta veterinaria Hungarica 农林科学-兽医学
CiteScore
1.80
自引率
0.00%
发文量
39
审稿时长
>36 weeks
期刊介绍: Acta Veterinaria Hungarica publishes original research papers presenting new scientific results of international interest, and to a limited extent also review articles and clinical case reports, on veterinary physiology (physiological chemistry and metabolism), veterinary microbiology (bacteriology, virology, immunology, molecular biology), on the infectious diseases of domestic animals, on veterinary parasitology, pathology, clinical veterinary science and reproduction.
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