Prevalence of carbapenemases in Enterobacterales from urine specimens in an university hospital in Istanbul, Turkey.

IF 1.3 4区医学 Q4 IMMUNOLOGY

Acta microbiologica et immunologica Hungarica Pub Date : 2023-06-16 DOI:10.1556/030.2023.01986

Münevver Sadunoğlu Güler, Gökhan Aygün, Seher Akkuş, Ahmet Mert Kuşkucu, Ömer Küçükbasmacİ, Nevrİye Gönüllü

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引用次数: 0

Abstract

We aimed to investigate the prevalence of carbapenemases in Enterobacterales strains isolated from urine specimens between July 2019 and July 2020.CIM and modified CIM tests were applied as well as detection of blaOXA-48, blaNDM, blaVIM, blaKPC and blaIMP genes was performed by multiplex PCR.One hundred fifty of 3,242 Enterobacterales strains were found to be carbapenem resistant and 46 were included in the study. Forty five (98%) of the 46 strains included in the study were Klebsiella spp. and one (2%) of them was Escherichia coli. Susceptibility to ceftazidime-avibactam, amikacin and gentamicin was 97%, 11% and 9%, respectively. Forty three (94%) isolates were found positive at 2 and 4 h with CIM test. Forty four (97%) strains were found positive at 4 h and 43 (94%) strains were found positive at 2 h with modified CIM test.While blaOXA-48, blaNDM and blaOXA-48 with blaNDM association were found in Klebsiella spp. isolates in 55%, 27% and 11%, respectively, blaVIM, blaKPC, blaIMP were not found. Only blaOXA-48 and blaNDM-1 were detected in the E. coli strain.None of the investigated genes were detected in three Klebsiella strains but with whole genome analysis the combination of blaOXA-534, blaCMY-99 and blaKPC-3 was found in the first strain, blaOXA-370 in the second strain and no resistance gene was found in the third strain.Ceftazidime-avibactam was found to be active against 97% of strains, and the most common resistance genes were blaOXA-48 and blaNDM-1. Previously undetected resistance genes have been identified in our country.

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土耳其伊斯坦布尔一所大学医院尿液标本中肠杆菌中碳青霉烯酶的流行情况。

我们的目的是调查2019年7月至2020年7月从尿液标本中分离的肠杆菌菌株中碳青霉烯酶的流行情况。采用CIM和改良CIM检测，多重PCR检测blaxa -48、blaNDM、blaVIM、blaKPC和blaIMP基因。在3242株肠杆菌中发现150株对碳青霉烯类耐药，其中46株纳入研究。46株病原菌中45株(98%)为克雷伯氏菌，1株(2%)为大肠杆菌。对头孢他啶-阿维巴坦、阿米卡星和庆大霉素的敏感性分别为97%、11%和9%。43株(94%)在2和4 h的CIM试验中呈阳性。改良CIM试验4 h阳性44株(97%)，2 h阳性43株(94%)。克雷伯菌分离株中blaOXA-48、blaNDM和blaOXA-48与blaNDM的相关性分别为55%、27%和11%，blaVIM、blaKPC和blaIMP未检出。大肠杆菌株中仅检测到blaOXA-48和blaNDM-1。3株克雷伯菌均未检出上述基因，但经全基因组分析，在1株克雷伯菌中发现blaOXA-534、blaCMY-99和blaKPC-3的组合，在2株克雷伯菌中发现blaOXA-370，在3株克雷伯菌中未发现耐药基因。头孢他啶-阿维巴坦对97%的菌株有活性，最常见的耐药基因为blaOXA-48和blaNDM-1。以前未被发现的耐药基因在我国已被发现。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta microbiologica et immunologica Hungarica IMMUNOLOGY-MICROBIOLOGY

CiteScore

2.30

自引率

13.30%

发文量

审稿时长

>12 weeks

期刊介绍： AMIH is devoted to the publication of research in all fields of medical microbiology (bacteriology, virology, parasitology, mycology); immunology of infectious diseases and study of the microbiome related to human diseases.